7W5P

Crystal Structure of the dioxygenase CcTet from Coprinopsis cinereain bound to 12bp N6-methyldeoxyadenine (6mA) containing duplex DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	104.788, 105.198, 196.717
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.180 - 2.300
R-factor	0.2292
Rwork	0.228
R-free	0.25730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vpn
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.111	0.084	0.726
Rmeas	0.116	0.088	0.753
Rpim	0.032	0.026	0.198
Total number of observations	1309069
Number of reflections	96239	5098	4767
<I/σ(I)>	4.4
Completeness [%]	99.5	98.6	99.8
Redundancy	13.6	12.2	14.1
CC(1/2)		0.955	0.863

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.1 M MES, pH 6.5, 20% PEG 8000