7W41
Crystal Structure of Human Gastrin Releasing Peptide Receptor in complex with the antagonist PD176252
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0330 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.104, 60.025, 98.489 |
| Unit cell angles | 90.00, 113.57, 90.00 |
Refinement procedure
| Resolution | 27.767 - 2.952 |
| Rwork | 0.280 |
| R-free | 0.32050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7f6g |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.767 | 3.060 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.151 | 0.599 |
| Number of reflections | 14852 | 1449 |
| <I/σ(I)> | 8.14 | |
| Completeness [%] | 96.9 | |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6.8 | 294 | 100mM MES, pH 6.8, 300mM Ammonium dihydrogen phosphate, 28% PEG 400, 5% polypropylene glycol P 400 |
