7VYT

Crystal structure of human TIGIT(23-129) in complex with the scFv fragment of anti-TIGIT antibody MG1131

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97957
Spacegroup name	P 1 21 1
Unit cell lengths	69.670, 79.820, 77.970
Unit cell angles	90.00, 100.55, 90.00

Refinement procedure

Resolution	29.330 - 1.530
R-factor	0.2021
Rwork	0.201
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ehv 3ucr
RMSD bond length	0.012
RMSD bond angle	1.196
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.330	1.585
High resolution limit [Å]	1.530	1.530
Rmerge	0.065
Number of reflections	126325	12553
<I/σ(I)>	16
Completeness [%]	99.9
Redundancy	7
CC(1/2)	0.999	0.703

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	200mM ammonium citrate, 20% (v/v) polyethylene glycol 3350