7VPQ

Structures of a deltacoronavirus spike protein bound to porcine and human receptors indicate the risk of virus adaptation to humans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979150
Spacegroup name	C 2 2 2
Unit cell lengths	201.225, 347.690, 255.114
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.360 - 3.100
R-factor	0.2601
Rwork	0.259
R-free	0.27840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6atk
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.290
High resolution limit [Å]	3.100	3.100
Number of reflections	160248	25301
<I/σ(I)>	5.28
Completeness [%]	99.4
Redundancy	6.1
CC(1/2)	0.964	0.583

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	solution containing 25% w/v Polyethylene glycol 1500, 0.1 M BIS-TRIS propane pH9.0, 0.1 M Sodium chloride