7VLN
NSD2-PWWP1 domain bound with an imidazol-5-yl benzonitrile compound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-12-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 70.120, 70.120, 230.670 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.364 - 3.090 |
| R-factor | 0.294329894931 |
| Rwork | 0.292 |
| R-free | 0.34524 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6xcg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.337 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.364 | 3.170 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Rpim | 0.043 | 0.442 |
| Number of reflections | 12759 | 912 |
| <I/σ(I)> | 18.9 | |
| Completeness [%] | 99.8 | |
| Redundancy | 19.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1.6 M ammonium sulfate, 0.01 M magnesium chloride, 0.1 M HEPES, pH 7.5 |
