7V8G

Crystal structure of HOIP RING1 domain bound to IpaH1.4 LRR domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	136.896, 35.141, 145.871
Unit cell angles	90.00, 93.69, 90.00

Refinement procedure

Resolution	60.360 - 2.750
R-factor	0.2233
Rwork	0.220
R-free	0.27810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5edv
RMSD bond length	0.014
RMSD bond angle	1.995
Data reduction software	XDS (20190606)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.780	2.900
High resolution limit [Å]	2.750	2.750
Rpim	0.050	0.168
Number of reflections	18572	2667
<I/σ(I)>	11	5.1
Completeness [%]	99.9	99.5
Redundancy	12.8	13.8
CC(1/2)	0.995	0.963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8	289	2%(v/v) 1,4-dioxane, 0.1 M Tris-HCl (pH 8.0), 15%(v/v) PEG 3,350