7UXD

Crystal structure of APOBEC3G Catalytic domain complex with ssDNA containing 2'-deoxy Zebularine.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	47.489, 47.121, 52.042
Unit cell angles	90.00, 103.18, 90.00

Refinement procedure

Resolution	34.506 - 1.500
R-factor	0.1736
Rwork	0.172
R-free	0.19440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6BUX (Chain A)
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.550
High resolution limit [Å]	1.500	3.230	1.500
Rmerge	0.086	0.079	0.468
Rmeas	0.104	0.095	0.561
Rpim	0.058	0.053	0.306
Total number of observations	106388
Number of reflections	33649	3094	3566
<I/σ(I)>	7.7
Completeness [%]	93.4	83.5	98.9
Redundancy	3.2	3.1	3.2
CC(1/2)		0.967	0.752

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	20% PEG6000, 50 mM di-Sodium L-Malate; pH5.0, 30 mM CACL2