7USZ
Human DDAH-1, holo (Zn-bound) form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.968, 47.634, 146.965 |
Unit cell angles | 90.00, 94.61, 90.00 |
Refinement procedure
Resolution | 29.550 - 1.650 |
R-factor | 0.1532 |
Rwork | 0.151 |
R-free | 0.19940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jaj |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.900 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmeas | 0.094 | |
Rpim | 0.036 | 0.531 |
Number of reflections | 73638 | 2000 |
<I/σ(I)> | 11.2 | 1.8 |
Completeness [%] | 98.3 | |
Redundancy | 6.8 | |
CC(1/2) | 0.998 | 0.704 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 100 mM Tris-HCl (pH 8.05), 21% PEG6000 |