7UJV
Structure of PHD2 in complex with HIF2a-CODD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-28 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97933 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 129.078, 37.597, 42.148 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.183 - 1.800 |
Rwork | 0.173 |
R-free | 0.21340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5l9b |
RMSD bond length | 0.006 |
RMSD bond angle | 1.368 |
Data reduction software | DIALS (2.1) |
Data scaling software | DIALS (2.1) |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 129.080 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.117 | 0.790 |
Rmeas | 0.126 | 0.860 |
Rpim | 0.047 | 0.334 |
Number of reflections | 19834 | 967 |
<I/σ(I)> | 8.4 | 0.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.1 | 6.5 |
CC(1/2) | 0.998 | 0.695 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 32.5% (w/v) PEG 2000 MME |