7U8F

Ternary complex structure of Cereblon-DDB1 bound to IKZF2(ZF2) and the molecular glue DKY709

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 61 2 2
Unit cell lengths	180.892, 180.892, 555.884
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.890 - 3.150
R-factor	0.2142
Rwork	0.213
R-free	0.24330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6h0f
Data reduction software	autoPROC
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.890	49.890	3.200
High resolution limit [Å]	3.150	17.250	3.150
Rmerge	0.247	0.045	2.811
Rmeas	0.255	0.046	2.900
Rpim	0.062	0.012	0.708
Total number of observations	1537361	9326	74659
Number of reflections	93823	696	4549
<I/σ(I)>	11.9	41.2	1.3
Completeness [%]	100.0	95.9	100
Redundancy	16.4	13.4	16.4
CC(1/2)	0.998	0.999	0.393

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	1.6 M potassium/sodium phosphate, pH 6.5