7TV4
Crystal structure of NEMO CoZi in complex with HOIP NZF1 and linear diubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 55.950, 69.560, 180.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.010 - 4.200 |
| R-factor | 0.2254 |
| Rwork | 0.222 |
| R-free | 0.28580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4owf |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.497 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.010 | 4.700 |
| High resolution limit [Å] | 4.200 | 4.200 |
| Number of reflections | 5542 | 1543 |
| <I/σ(I)> | 9.5 | 5.8 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 13.1 | 12.9 |
| CC(1/2) | 0.830 | 0.420 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris-HCl, pH 8.5, 22% v/v PEG Smear Broad |
