7TTZ

Heterodimeric IgA Fc in complex with Staphylococcus aureus protein SSL7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	135.505, 165.447, 46.434
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.560 - 2.350
R-factor	0.2025
Rwork	0.200
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qej
RMSD bond length	0.008
RMSD bond angle	1.014
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.560	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.120	1.508
Number of reflections	48763	3864
<I/σ(I)>	14.8	2.15
Completeness [%]	98.6
Redundancy	2
CC(1/2)	1.000	0.639

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	30% PEG MME 550, 0.1 M HEPES pH 7.5, 0.2 Magnesium chloride