7TPB
p120RasGAP SH3 domain in complex with DLC1 RhoGAP domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-11 |
Detector | DECTRIS EIGER2 S 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | H 3 |
Unit cell lengths | 143.745, 143.745, 152.776 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.310 |
High resolution limit [Å] | 3.200 | 6.890 | 3.200 |
Rmerge | 0.256 | 0.122 | 1.700 |
Rmeas | 0.272 | 0.130 | 1.824 |
Rpim | 0.090 | 0.042 | 0.657 |
Total number of observations | 179535 | ||
Number of reflections | 19702 | 1966 | 1952 |
<I/σ(I)> | 5.3 | ||
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 9.1 | 9.2 | 7.8 |
CC(1/2) | 0.992 | 0.332 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.2 M Magnesium nitrate, 16.5% PEG 3350 |