7TFF

Crystal structure of human platelet phosphofructokinase-1 mutant- D564N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	77
Detector technology	PIXEL
Collection date	2021-10-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	77.889, 159.655, 131.248
Unit cell angles	90.00, 104.01, 90.00

Refinement procedure

Resolution	66.570 - 3.600
R-factor	0.2536
Rwork	0.250
R-free	0.30670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xyk
RMSD bond length	0.001
RMSD bond angle	0.346
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	161.740	3.760
High resolution limit [Å]	3.600	3.600
Rmerge	0.211	1.153
Rmeas	0.250	1.377
Rpim	0.132	0.742
Number of reflections	36675	4395
<I/σ(I)>	6.2	1.2
Completeness [%]	98.7	97
Redundancy	6.4
CC(1/2)	0.988

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5.5	295.15	125uM ATP, 25% PEG3350, 0.2M ammonium sulfate, 0.1M Bis-Tris pH 5.5