7TFF

Crystal structure of human platelet phosphofructokinase-1 mutant- D564N

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	ATP-dependent 6-phosphofructokinase, platelet type	polymer	812	89143.7	4	UniProt (Q01813) Pfam (PF00365)	Homo sapiens (human)	ATP-PFK,PFK-P,6-phosphofructokinase type C,Phosphofructo-1-kinase isozyme C,PFK-C,Phosphohexokinase
2	E, F, K, L, O... (A, B, C, D)	PHOSPHATE ION	non-polymer		95.0	8	Chemie (PO4)
3	G, H, I, J, M... (A, B, C, D)	SULFATE ION	non-polymer		96.1	13	Chemie (SO4)
4	AA, BA, CA, Z (B, C, D, A)	water	water		18.0	64	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 784 (UniProt: Q01813)

PDB	External Database	Details
Met -27	-	expression tag
Ser -26	-	expression tag
Tyr -25	-	expression tag
Tyr -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
Asp -17	-	expression tag
Tyr -16	-	expression tag
Asp -15	-	expression tag
Ile -14	-	expression tag
Pro -13	-	expression tag
Thr -12	-	expression tag
Thr -11	-	expression tag
Glu -10	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Ala -3	-	expression tag
Met -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag
Asn 564	Asp 564	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	356575.0
Non-Polymers*	Number of molecules	21
	Total formula weight	2008.6
All*	Total formula weight	358583.6

*Water molecules are not included.