7T2L

Crystal Structure of TEAD2 in a covalent complex with TED-662

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2020-09-24
Detector	RDI CMOS_8M
Wavelength(s)	1.00003
Spacegroup name	C 1 2 1
Unit cell lengths	121.651, 61.746, 79.753
Unit cell angles	90.00, 117.33, 90.00

Refinement procedure

Resolution	53.610 - 2.150
R-factor	0.2058
Rwork	0.203
R-free	0.25660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6e5g
RMSD bond length	0.006
RMSD bond angle	0.798
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.610	2.490
High resolution limit [Å]	2.150	2.150
Rmeas	0.107	1.088
Rpim	0.055	0.579
Number of reflections	14087	704
<I/σ(I)>	10.6	1.3
Completeness [%]	87.7	62.5
Redundancy	3.7	3.3
CC(1/2)	0.997	0.485

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Sodium Formate 1.8M - 2.4M HEPES, pH 7.2-7.4