7ST5

Structure of Fab CC-95251 in complex with SIRP-alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-11
Detector	RIGAKU SATURN 92
Wavelength(s)	1.0782
Spacegroup name	P 1
Unit cell lengths	42.957, 97.704, 103.385
Unit cell angles	77.18, 85.92, 85.74

Refinement procedure

Resolution	50.010 - 2.200
R-factor	0.2463
Rwork	0.245
R-free	0.27970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jjs
RMSD bond length	0.016
RMSD bond angle	1.764
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.000	2.220
High resolution limit [Å]	2.100	2.110
Number of reflections	83446	12639
<I/σ(I)>	6.3	2.3
Completeness [%]	88.6
Redundancy	2.2
CC(1/2)	0.996	0.804

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1M MES pH 6.5, 25 %w/v PEG MME 550, 0.01 M Zinc sulfate