7SR9
Human alpha-thrombin with 180- and 220- loops replaced with homologous loops from protein C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU R-AXIS IV |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-11-08 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.969, 78.525, 49.602 |
| Unit cell angles | 90.00, 103.22, 90.00 |
Refinement procedure
| Resolution | 30.460 - 2.100 |
| R-factor | 0.1806 |
| Rwork | 0.178 |
| R-free | 0.22741 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ppb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.393 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 21066 | 21066 |
| <I/σ(I)> | 14.2 | |
| Completeness [%] | 96.9 | |
| Redundancy | 3.02 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate, 30% PEG4000 |
