7SQ2

Reprocessed and refined structure of Phospholipase C-beta and Gq signaling complex

Replaces:  3OHM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.000
Spacegroup name	C 1 2 1
Unit cell lengths	202.837, 90.817, 93.135
Unit cell angles	90.00, 101.19, 90.00

Refinement procedure

Resolution	40.550 - 2.600
R-factor	0.20247
Rwork	0.199
R-free	0.26334
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3OHM
RMSD bond length	0.003
RMSD bond angle	1.227
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.550	2.666
High resolution limit [Å]	2.600	2.600
Rmeas	0.084	0.723
Rpim	0.035	0.304
Number of reflections	48454	2555
<I/σ(I)>	32.5	2.67
Completeness [%]	99.5	99.6
Redundancy	5.6	5.5
CC(1/2)	0.905	0.905

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	7% PEG 6000, 100 mM Hepes, 1 mM CaCl2, 100 mM magnesium acetate