7SJP

anti-HtrA1 Fab15H6.v4 bound to HtrA1-LoopA peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	93
Detector technology	PIXEL
Collection date	2018-01-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	60.193, 90.212, 106.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.450 - 2.100
R-factor	0.1698
Rwork	0.168
R-free	0.19880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7sjm
RMSD bond length	0.010
RMSD bond angle	1.140
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	BUSTER (2.11.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.450	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.107	0.814
Rmeas	0.117
Rpim	0.062	0.547
Number of reflections	34460	2691
<I/σ(I)>	12.7	1.8
Completeness [%]	99.7	96.4
Redundancy	6.4	4.4
CC(1/2)	0.998	0.727

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	12% PEG 4000, 10% Ethanol, 5% glycerol