7RH2

IRF4 Transcription factor mutant -K59R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2021-02-10
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9537
Spacegroup name	P 31 2 1
Unit cell lengths	114.874, 114.874, 154.788
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.120 - 2.470
R-factor	0.2052
Rwork	0.203
R-free	0.24050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jm4
RMSD bond length	0.011
RMSD bond angle	1.286
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.360	2.570
High resolution limit [Å]	2.470	2.470
Rmerge	0.072	0.850
Rmeas	0.075
Rpim	0.033	0.380
Number of reflections	42630	4323
<I/σ(I)>	15.4	1.7
Completeness [%]	99.8	97.5
Redundancy	10.2	10.2
CC(1/2)	0.998	0.832

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	3-9 % PEG 4000, 0.1M Na acetate pH 4.8