7RE7

TCR mimic antibody (Fab fragment) in complex with AFP/HLA-A*02

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	49.707, 200.912, 244.574
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.201 - 2.547
R-factor	0.1837
Rwork	0.180
R-free	0.21960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e00
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.580
High resolution limit [Å]	2.540	2.540
Number of reflections	81215	3257
<I/σ(I)>	19.9
Completeness [%]	99.3
Redundancy	12.4
CC(1/2)	0.994	0.764

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	296.15	18% v/v PEG 3350 and 0.2 M sodium formate solution, pH 7.2