7RDT

Structure of human NTHL1 - linker 1 chimera

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	42.370, 71.680, 86.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.050 - 2.100
R-factor	0.1861
Rwork	0.181
R-free	0.23060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rds
RMSD bond length	0.002
RMSD bond angle	0.471
Data reduction software	MOSFLM
Data scaling software	pointless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.050	2.160
High resolution limit [Å]	2.100	2.100
Rpim	0.053	0.210
Number of reflections	28900	1269
<I/σ(I)>	8.9	3.4
Completeness [%]	100.0	99.5
Redundancy	4.1	4
CC(1/2)	0.995	0.843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	291	PEG 6K, Sodium chloride, Tricine,