7R2V
Structure of nsp14 from SARS-CoV-2 in complex with SAH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-01 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.176, 100.312, 90.874 |
Unit cell angles | 90.00, 107.66, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.530 |
R-factor | 0.1991 |
Rwork | 0.196 |
R-free | 0.25430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7qgi |
RMSD bond length | 0.003 |
RMSD bond angle | 1.254 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 86.600 | 2.885 |
High resolution limit [Å] | 2.530 | 2.532 |
Rmerge | 0.160 | 0.914 |
Number of reflections | 19046 | 2830 |
<I/σ(I)> | 6.8 | |
Completeness [%] | 87.8 | |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 278 | PEG/Imidazole |