7QUH
Siglec-8 in complex with therapeutic Fab AK002.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-29 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.00003 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 145.343, 44.554, 111.387 |
Unit cell angles | 90.00, 111.41, 90.00 |
Refinement procedure
Resolution | 42.319 - 2.867 |
R-factor | 0.2277 |
Rwork | 0.225 |
R-free | 0.28360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7qu6 |
Data reduction software | autoPROC |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.320 | 2.970 |
High resolution limit [Å] | 2.867 | 2.870 |
Rmerge | 0.233 | 1.042 |
Rpim | 0.097 | 0.446 |
Number of reflections | 14935 | 1575 |
<I/σ(I)> | 9.3 | |
Completeness [%] | 95.4 | |
Redundancy | 6.7 | |
CC(1/2) | 0.985 | 0.678 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M ammonium chloride 20% PEG3350 pH6.3 |