7QDN
Structure of human liver pyruvate kinase from which the B domain has been deleted
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-30 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.91589 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 207.448, 112.687, 188.333 |
| Unit cell angles | 90.00, 91.36, 90.00 |
Refinement procedure
| Resolution | 103.690 - 1.695 |
| R-factor | 0.2059 |
| Rwork | 0.205 |
| R-free | 0.21710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ip7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.900 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.695 | 1.725 |
| High resolution limit [Å] | 1.695 | 1.695 |
| Rmerge | 0.095 | 4.784 |
| Number of reflections | 447443 | 22972 |
| <I/σ(I)> | 14.5 | 0.5 |
| Completeness [%] | 93.5 | 96.3 |
| Redundancy | 15.3 | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 100 mM HEPES/MOPS, 10% PEG8000, 20% ethylene glycol, 10 mM phenylalanine, 20 mM sodium oxalate |
