7Q9B

MHC Class I A02 Allele presenting EAAGIGILTV, in complex with Mel8 TCR

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-17
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97625
Spacegroup name	P 1 21 1
Unit cell lengths	99.972, 53.645, 203.507
Unit cell angles	90.00, 94.33, 90.00

Refinement procedure

Resolution	99.686 - 3.240
Rwork	0.226
R-free	0.27650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hg1
RMSD bond length	0.011
RMSD bond angle	1.452
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.700	3.320
High resolution limit [Å]	3.240	3.240
Rmerge	0.280	0.949
Rmeas	0.328	1.102
Rpim	0.220	0.719
Number of reflections	34992	2579
<I/σ(I)>	2.7
Completeness [%]	100.0	99.9
Redundancy	3.7	3.8
CC(1/2)	0.995	0.686

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293