7Q5W
The tandem SH2 domains of SYK with a bound TYROBP diphospho-ITAM peptide
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-27 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 117.949, 131.928, 141.526 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 65.960 - 2.200 |
Rwork | 0.234 |
R-free | 0.26630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a81 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.420 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.960 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.132 | 4.191 |
Rmeas | 0.137 | 4.259 |
Rpim | 0.039 | 1.195 |
Number of reflections | 112454 | 5513 |
<I/σ(I)> | 14 | 0.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 24.2 | 25.8 |
CC(1/2) | 0.999 | 0.571 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 30 mM sodium nitrate, 30 mM dibasic sodium phosphate, 30 mM ammonium sulphate, 100 mM Tris/BICINE, 10% PEG 20,000, 20% PEG 500 MME |