7PQ0
Crystal structure of the Burkholderia Lethal Factor 1 (BLF1) C94S inactive mutant in complex with human eIF4A - Crystal form B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-03 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 63 |
| Unit cell lengths | 199.530, 199.530, 51.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.310 - 3.000 |
| R-factor | 0.1883 |
| Rwork | 0.186 |
| R-free | 0.22980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tua 2zu6 2g9n |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.551 |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.5.23) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 65.310 | 65.310 | 3.080 |
| High resolution limit [Å] | 3.000 | 13.420 | 3.000 |
| Rmerge | 0.041 | 0.029 | 0.216 |
| Rmeas | 0.044 | 0.032 | 0.230 |
| Rpim | 0.015 | 0.013 | 0.078 |
| Total number of observations | 198942 | 2006 | 14304 |
| Number of reflections | 23808 | 301 | 1702 |
| <I/σ(I)> | 34.9 | 67.9 | 9 |
| Completeness [%] | 99.7 | 98.9 | 97.9 |
| Redundancy | 8.4 | 6.7 | 8.4 |
| CC(1/2) | 0.999 | 0.997 | 0.988 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 0.1 M HEPES, 4 % (w/v) PEG 6000 |
