7PI4

FAK Protac GSK215 in complex with FAK and pVHL:ElonginC:ElonginB

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-11
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	59.550, 77.260, 175.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.381 - 2.240
Rwork	0.195
R-free	0.25120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3psk
RMSD bond length	0.007
RMSD bond angle	1.431
Data reduction software	xia2
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.390	2.300
High resolution limit [Å]	2.240	2.240
Rmerge	0.198	0.978
Number of reflections	39778	2918
<I/σ(I)>	6.2	2.2
Completeness [%]	99.9	99.9
Redundancy	6.7	6.9
CC(1/2)	0.984	0.779

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.05M CaCl, 0.1M MES pH 6.5, 45%v/v, PEG200