7PHS

highly potent IL6 antagonistic antibody selected from a camelid immune phage display repertoire

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-07-27
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.923
Spacegroup name	P 43 21 2
Unit cell lengths	58.990, 58.990, 285.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.050 - 2.770
R-factor	0.2763
Rwork	0.273
R-free	0.30280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a8j 3b2u
RMSD bond length	0.008
RMSD bond angle	1.280
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER (2.11.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.900
High resolution limit [Å]	2.770	2.770
Rmerge	0.110	0.860
Number of reflections	13561	1635
<I/σ(I)>	18.6	3.3
Completeness [%]	98.4
Redundancy	8.6
CC(1/2)	0.990	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.5	293	30% PEG 8K, 0.2M Li2SO4, 0.1M acetate pH 4.5.