7OZT

Nanobodies restore stability to cancer-associated mutants of tumor suppressor protein p16INK4a

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97622
Spacegroup name	C 2 2 21
Unit cell lengths	42.119, 182.931, 65.225
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	91.465 - 1.740
Rwork	0.180
R-free	0.20860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bi7 3ogo
RMSD bond length	0.016
RMSD bond angle	2.081
Data reduction software	DIALS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	91.470	91.470	1.770
High resolution limit [Å]	1.740	9.040	1.740
Rmerge	0.101	0.046	0.878
Rmeas	0.119	0.055	1.058
Rpim	0.061	0.029	0.582
Number of reflections	26446	239	1412
<I/σ(I)>	9.2
Completeness [%]	100.0
Redundancy	6.8	5.6	6.2
CC(1/2)	0.998	0.999	0.361

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Molecular Dimensions Morpheus screen condition G4