7OZT

Nanobodies restore stability to cancer-associated mutants of tumor suppressor protein p16INK4a

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (AAA)	Camelid nanobody NB09	polymer	132	14208.8	1		Lama glama (Llama)
2	B (BBB)	Cyclin-dependent kinase inhibitor 2A	polymer	175	18701.0	1	UniProt (P42771)	Homo sapiens (Human)	Cyclin-dependent kinase 4 inhibitor A,CDK4I,Multiple tumor suppressor 1,MTS-1,p16-INK4a,p16-INK4,p16INK4A
3	C, D (AAA, BBB)	water	water		18.0	90	Chemie (HOH)

Sequence modifications

BBB: 1 - 156 (UniProt: P42771)

PDB	External Database	Details
Met -18	-	initiating methionine
Ala -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	32909.8
All*	Total formula weight	32909.8

*Water molecules are not included.