7OY1
DnrK mutant RTCR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.400, 105.690, 64.450 |
| Unit cell angles | 90.00, 111.18, 90.00 |
Refinement procedure
| Resolution | 49.700 - 2.390 |
| R-factor | 0.2293 |
| Rwork | 0.227 |
| R-free | 0.26680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tw2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.179 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.710 | 2.480 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.242 | |
| Rmeas | 0.138 | |
| Rpim | 0.169 | |
| Number of reflections | 29636 | 2854 |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 99.1 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2 M Magnesium chloride hexahydrate; 0.1 M HEPES 7.0; 20 % w/v PEG 6000 |
