7OPQ
Rab27a fusion with Slp2a-RBDa1 effector covalent adduct with CA1 in C188
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-30 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.710, 76.816, 117.818 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.520 - 2.230 |
R-factor | 0.1794 |
Rwork | 0.177 |
R-free | 0.22390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bc1 |
Data reduction software | DIALS |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.520 | 2.310 |
High resolution limit [Å] | 2.230 | 2.230 |
Rmerge | 0.034 | 0.360 |
Rmeas | 0.048 | 0.509 |
Rpim | 0.034 | 0.360 |
Number of reflections | 54026 | 2005 |
<I/σ(I)> | 13.67 | 2.01 |
Completeness [%] | 96.9 | 72.64 |
Redundancy | 2 | 2 |
CC(1/2) | 0.998 | 0.760 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277.15 | 0.15M Ammonium sulphate, 0.1M MES pH 6.0, 15% w/v PEG 4000 |