7OPO
RSK2 N-terminal kinase domain in complex with ORF45
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 170.290, 88.621, 160.346 |
| Unit cell angles | 90.00, 95.42, 90.00 |
Refinement procedure
| Resolution | 47.650 - 2.750 |
| R-factor | 0.2094 |
| Rwork | 0.209 |
| R-free | 0.23220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g51 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.899 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.650 | 47.650 | 2.820 |
| High resolution limit [Å] | 2.750 | 12.300 | 2.750 |
| Rmerge | 0.120 | 0.028 | 1.598 |
| Rmeas | 0.130 | 0.030 | 1.724 |
| Rpim | 0.048 | 0.012 | 0.642 |
| Total number of observations | 437614 | 4719 | 32437 |
| Number of reflections | 61675 | 726 | 4548 |
| <I/σ(I)> | 11.5 | 49.9 | 1.3 |
| Completeness [%] | 99.5 | 97.9 | 99.2 |
| Redundancy | 7.1 | 6.5 | 7.1 |
| CC(1/2) | 0.998 | 0.999 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 12 %PEG 6000, HEPES pH 7.5, 0.75M NaCl in reservoir |
