7OLY

Structure of activin A in complex with an ActRIIB-Alk4 fusion reveal insight into activin receptor interactions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9920
Spacegroup name	P 61 2 2
Unit cell lengths	68.375, 68.375, 975.538
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.250 - 3.265
R-factor	0.2362
Rwork	0.234
R-free	0.26850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5k59 5mac 2arv
RMSD bond length	0.008
RMSD bond angle	0.990
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.11.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.250	3.390
High resolution limit [Å]	3.260	3.260
Rmerge	0.317	2.667
Rpim	0.054	0.446
Number of reflections	23230	4489
<I/σ(I)>	11.7	1.9
Completeness [%]	99.9	100
Redundancy	35.3
CC(1/2)	0.999	0.504

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	17.7 mg/ml complex in 20 mM HEPES, 200 mM NaCl pH 7.5. 100 + 100 nl sitting drop in a MRC 3-well plate with reservoir 0.1 M Na cacodylate pH 6.5 and 17 % (w/v) PEG 4000).