7OLI

Crystal structure of Pab-AGOG in complex with 8-oxoguanosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97857
Spacegroup name	C 1 2 1
Unit cell lengths	131.063, 47.202, 91.271
Unit cell angles	90.00, 100.80, 90.00

Refinement procedure

Resolution	44.830 - 1.650
R-factor	0.1628
Rwork	0.161
R-free	0.19070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7olb
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.190	1.740
High resolution limit [Å]	1.650	1.650
Rmerge	0.081	0.404
Number of reflections	65858	9640
<I/σ(I)>	7.3	2.3
Completeness [%]	99.3
Redundancy	3.2
CC(1/2)	0.992	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	LiCl, tri-Na citrate, PEG 6000