7OKP

Crystal structure of mouse CARM1 in complex with histone H3_13-22 K18 acetylated

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-X
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-24
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	74.909, 99.580, 208.075
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.320 - 2.200
R-factor	0.213
Rwork	0.211
R-free	0.25430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ih3
Data reduction software	XDS
Data scaling software	Aimless (1.12.8)
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.110	2.250
High resolution limit [Å]	2.200	2.200
Rmerge	0.092	0.693
Rmeas	0.101	0.809
Rpim	0.040	0.406
Number of reflections	77281	4317
<I/σ(I)>	9.3	1.5
Completeness [%]	97.1	93.7
Redundancy	5.3	3.8
CC(1/2)	0.998	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1M sodium malonate, 0.1M MES pH7.0, 0.2M sodium chloride