7OGS

X-ray Structure of Interferon Regulatory Factor 4 DNA binding domain bound to an interferon-stimulated response element

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-26
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9119
Spacegroup name	P 21 21 21
Unit cell lengths	64.347, 66.082, 202.195
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.810 - 2.370
R-factor	0.2183
Rwork	0.217
R-free	0.24940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7o56
RMSD bond length	0.010
RMSD bond angle	1.251
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.350	2.410
High resolution limit [Å]	2.370	2.370
Rmerge	0.174	3.086
Rmeas	0.189	3.409
Rpim	0.074	1.434
Number of reflections	35957	1740
<I/σ(I)>	5.8	0.2
Completeness [%]	100.0	100
Redundancy	6.3	5.5
CC(1/2)	0.998	0.281

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1M Bis Tris pH 5.5, 0.2M ammonium acetate, 25% PEG 3350