7OAT
Structural basis for targeted p97 remodelling by ASPL as prerequisite for p97 trimethylation by METTL21D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.258, 69.622, 140.175 |
| Unit cell angles | 90.00, 94.41, 90.00 |
Refinement procedure
| Resolution | 49.320 - 2.995 |
| R-factor | 0.2275 |
| Rwork | 0.225 |
| R-free | 0.27040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ifs |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.400 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3 (6-FEB-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.320 | 3.150 |
| High resolution limit [Å] | 2.995 | 3.000 |
| Rmeas | 0.273 | 1.983 |
| Number of reflections | 20828 | 3167 |
| <I/σ(I)> | 6.65 | 0.79 |
| Completeness [%] | 98.1 | |
| Redundancy | 6.17 | |
| CC(1/2) | 0.986 | 0.324 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 16% PEG 8000, 0.2 M calcium acetate, 0.1 M MES (pH 6.5) |
