7O3L
Crystal Structure of AcrB Double Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-16 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.97950 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 152.154, 156.722, 218.751 |
Unit cell angles | 90.00, 92.40, 90.00 |
Refinement procedure
Resolution | 19.932 - 3.526 |
R-factor | 0.2686 |
Rwork | 0.264 |
R-free | 0.35430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gif |
RMSD bond length | 0.047 |
RMSD bond angle | 2.004 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 98.880 | 3.720 |
High resolution limit [Å] | 3.526 | 3.526 |
Rpim | 0.097 | 0.490 |
Number of reflections | 126421 | 18381 |
<I/σ(I)> | 7.1 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 8.9 | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.1 M MES PH 6.5, 0.2 M MgAC, 10% PEG 3350 |