Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7O3L

Crystal Structure of AcrB Double Mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005515	molecular_function	protein binding
A	0005886	cellular_component	plasma membrane
A	0015562	molecular_function	efflux transmembrane transporter activity
A	0016020	cellular_component	membrane
A	0022857	molecular_function	transmembrane transporter activity
A	0042802	molecular_function	identical protein binding
A	0042908	biological_process	xenobiotic transport
A	0042910	molecular_function	xenobiotic transmembrane transporter activity
A	0055085	biological_process	transmembrane transport
A	0098567	cellular_component	periplasmic side of plasma membrane
A	0140330	biological_process	xenobiotic detoxification by transmembrane export across the cell outer membrane
A	1990281	cellular_component	efflux pump complex
B	0005515	molecular_function	protein binding
B	0005886	cellular_component	plasma membrane
B	0015562	molecular_function	efflux transmembrane transporter activity
B	0016020	cellular_component	membrane
B	0022857	molecular_function	transmembrane transporter activity
B	0042802	molecular_function	identical protein binding
B	0042908	biological_process	xenobiotic transport
B	0042910	molecular_function	xenobiotic transmembrane transporter activity
B	0055085	biological_process	transmembrane transport
B	0098567	cellular_component	periplasmic side of plasma membrane
B	0140330	biological_process	xenobiotic detoxification by transmembrane export across the cell outer membrane
B	1990281	cellular_component	efflux pump complex
C	0005515	molecular_function	protein binding
C	0005886	cellular_component	plasma membrane
C	0015562	molecular_function	efflux transmembrane transporter activity
C	0016020	cellular_component	membrane
C	0022857	molecular_function	transmembrane transporter activity
C	0042802	molecular_function	identical protein binding
C	0042908	biological_process	xenobiotic transport
C	0042910	molecular_function	xenobiotic transmembrane transporter activity
C	0055085	biological_process	transmembrane transport
C	0098567	cellular_component	periplasmic side of plasma membrane
C	0140330	biological_process	xenobiotic detoxification by transmembrane export across the cell outer membrane
C	1990281	cellular_component	efflux pump complex
D	0005515	molecular_function	protein binding
D	0005886	cellular_component	plasma membrane
D	0015562	molecular_function	efflux transmembrane transporter activity
D	0016020	cellular_component	membrane
D	0022857	molecular_function	transmembrane transporter activity
D	0042802	molecular_function	identical protein binding
D	0042908	biological_process	xenobiotic transport
D	0042910	molecular_function	xenobiotic transmembrane transporter activity
D	0055085	biological_process	transmembrane transport
D	0098567	cellular_component	periplasmic side of plasma membrane
D	0140330	biological_process	xenobiotic detoxification by transmembrane export across the cell outer membrane
D	1990281	cellular_component	efflux pump complex
E	0005515	molecular_function	protein binding
E	0005886	cellular_component	plasma membrane
E	0015562	molecular_function	efflux transmembrane transporter activity
E	0016020	cellular_component	membrane
E	0022857	molecular_function	transmembrane transporter activity
E	0042802	molecular_function	identical protein binding
E	0042908	biological_process	xenobiotic transport
E	0042910	molecular_function	xenobiotic transmembrane transporter activity
E	0055085	biological_process	transmembrane transport
E	0098567	cellular_component	periplasmic side of plasma membrane
E	0140330	biological_process	xenobiotic detoxification by transmembrane export across the cell outer membrane
E	1990281	cellular_component	efflux pump complex
F	0005515	molecular_function	protein binding
F	0005886	cellular_component	plasma membrane
F	0015562	molecular_function	efflux transmembrane transporter activity
F	0016020	cellular_component	membrane
F	0022857	molecular_function	transmembrane transporter activity
F	0042802	molecular_function	identical protein binding
F	0042908	biological_process	xenobiotic transport
F	0042910	molecular_function	xenobiotic transmembrane transporter activity
F	0055085	biological_process	transmembrane transport
F	0098567	cellular_component	periplasmic side of plasma membrane
F	0140330	biological_process	xenobiotic detoxification by transmembrane export across the cell outer membrane
F	1990281	cellular_component	efflux pump complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	binding site for residue LMT A 2000

Chain	Residue
A	SER530
A	GLY533
A	ILE534
A	ARG536
A	TYR541
A	VAL1016

site_id	AC2
Number of Residues	7
Details	binding site for residue LMT B 2000

Chain	Residue
B	ARG536
B	SER537
B	TYR541
B	PHE1020
B	SER530
B	GLY533
B	ILE534

site_id	AC3
Number of Residues	7
Details	binding site for residue LMT C 2000

Chain	Residue
C	SER530
C	GLY533
C	ILE534
C	ARG536
C	ARG540
C	TYR541
C	PHE1020

site_id	AC4
Number of Residues	7
Details	binding site for residue LMT D 2000

Chain	Residue
D	SER530
D	GLY533
D	ILE534
D	ARG536
D	ARG540
D	TYR541
D	PHE1020

site_id	AC5
Number of Residues	8
Details	binding site for residue LMT E 2000

Chain	Residue
E	SER530
E	GLY533
E	ILE534
E	ARG536
E	SER537
E	ARG540
E	TYR541
E	PHE1020

site_id	AC6
Number of Residues	8
Details	binding site for residue LMT F 2000

Chain	Residue
F	HIS526
F	SER530
F	GLY533
F	ILE534
F	ARG536
F	SER537
F	ARG540
F	TYR541

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	924
Details	TOPO_DOM: Cytoplasmic => ECO:0000269\|PubMed:15919996

Chain	Residue	Details
A	MET1-PRO9
A	LEU357-THR365
A	GLU414-ILE438
A	ALA491-THR538
A	ALA889-PRO898
A	LEU944-LEU972
A	ILE1019-HIS1049
B	MET1-PRO9
B	LEU357-THR365
B	GLU414-ILE438
B	ALA491-THR538
B	ALA889-PRO898
B	LEU944-LEU972
B	ILE1019-HIS1049
C	MET1-PRO9
C	LEU357-THR365
C	GLU414-ILE438
C	ALA491-THR538
C	ALA889-PRO898
C	LEU944-LEU972
C	ILE1019-HIS1049
D	MET1-PRO9
D	LEU357-THR365
D	GLU414-ILE438
D	ALA491-THR538
D	ALA889-PRO898
D	LEU944-LEU972
D	ILE1019-HIS1049
F	MET1-PRO9
F	LEU357-THR365
F	GLU414-ILE438
F	ALA491-THR538
F	ALA889-PRO898
F	LEU944-LEU972
F	ILE1019-HIS1049
E	MET1-PRO9
E	LEU357-THR365
E	GLU414-ILE438
E	ALA491-THR538
E	ALA889-PRO898
E	LEU944-LEU972
E	ILE1019-HIS1049

site_id	SWS_FT_FI2
Number of Residues	108
Details	TRANSMEM: Helical; Name=1

Chain	Residue	Details
A	ILE10-LEU28
B	ILE10-LEU28
C	ILE10-LEU28
D	ILE10-LEU28
F	ILE10-LEU28
E	ILE10-LEU28

site_id	SWS_FT_FI3
Number of Residues	3864
Details	TOPO_DOM: Periplasmic => ECO:0000269\|PubMed:15919996

Chain	Residue	Details
A	LYS29-SER336
A	PHE386-ASN391
A	PHE458-ALA465
A	PHE556-ASN871
A	ARG919-ASP924
A	THR993-GLY998
B	LYS29-SER336
B	PHE386-ASN391
B	PHE458-ALA465
B	PHE556-ASN871
B	ARG919-ASP924
B	THR993-GLY998
C	LYS29-SER336
C	PHE386-ASN391
C	PHE458-ALA465
C	PHE556-ASN871
C	ARG919-ASP924
C	THR993-GLY998
D	LYS29-SER336
D	PHE386-ASN391
D	PHE458-ALA465
D	PHE556-ASN871
D	ARG919-ASP924
D	THR993-GLY998
F	LYS29-SER336
F	PHE386-ASN391
F	PHE458-ALA465
F	PHE556-ASN871
F	ARG919-ASP924
F	THR993-GLY998
E	LYS29-SER336
E	PHE386-ASN391
E	PHE458-ALA465
E	PHE556-ASN871
E	ARG919-ASP924
E	THR993-GLY998

site_id	SWS_FT_FI4
Number of Residues	114
Details	TRANSMEM: Helical; Name=2

Chain	Residue	Details
A	ILE337-TYR356
B	ILE337-TYR356
C	ILE337-TYR356
D	ILE337-TYR356
F	ILE337-TYR356
E	ILE337-TYR356

site_id	SWS_FT_FI5
Number of Residues	114
Details	TRANSMEM: Helical; Name=3

Chain	Residue	Details
A	LEU366-ALA385
B	LEU366-ALA385
C	LEU366-ALA385
D	LEU366-ALA385
F	LEU366-ALA385
E	LEU366-ALA385

site_id	SWS_FT_FI6
Number of Residues	126
Details	TRANSMEM: Helical; Name=4

Chain	Residue	Details
A	THR392-VAL413
B	THR392-VAL413
C	THR392-VAL413
D	THR392-VAL413
F	THR392-VAL413
E	THR392-VAL413

site_id	SWS_FT_FI7
Number of Residues	108
Details	TRANSMEM: Helical; Name=5

Chain	Residue	Details
A	GLN439-ALA457
B	GLN439-ALA457
C	GLN439-ALA457
D	GLN439-ALA457
F	GLN439-ALA457
E	GLN439-ALA457

site_id	SWS_FT_FI8
Number of Residues	144
Details	TRANSMEM: Helical; Name=6

Chain	Residue	Details
A	ILE466-PRO490
B	ILE466-PRO490
C	ILE466-PRO490
D	ILE466-PRO490
F	ILE466-PRO490
E	ILE466-PRO490

site_id	SWS_FT_FI9
Number of Residues	96
Details	TRANSMEM: Helical; Name=7

Chain	Residue	Details
A	GLY539-LEU555
B	GLY539-LEU555
C	GLY539-LEU555
D	GLY539-LEU555
F	GLY539-LEU555
E	GLY539-LEU555

site_id	SWS_FT_FI10
Number of Residues	96
Details	TRANSMEM: Helical; Name=8

Chain	Residue	Details
A	GLN872-LEU888
B	GLN872-LEU888
C	GLN872-LEU888
D	GLN872-LEU888
F	GLN872-LEU888
E	GLN872-LEU888

site_id	SWS_FT_FI11
Number of Residues	114
Details	TRANSMEM: Helical; Name=9

Chain	Residue	Details
A	PHE899-PHE918
B	PHE899-PHE918
C	PHE899-PHE918
D	PHE899-PHE918
F	PHE899-PHE918
E	PHE899-PHE918

site_id	SWS_FT_FI12
Number of Residues	108
Details	TRANSMEM: Helical; Name=10

Chain	Residue	Details
A	VAL925-ILE943
B	VAL925-ILE943
C	VAL925-ILE943
D	VAL925-ILE943
F	VAL925-ILE943
E	VAL925-ILE943

site_id	SWS_FT_FI13
Number of Residues	114
Details	TRANSMEM: Helical; Name=11

Chain	Residue	Details
A	ARG973-SER992
B	ARG973-SER992
C	ARG973-SER992
D	ARG973-SER992
F	ARG973-SER992
E	ARG973-SER992

site_id	SWS_FT_FI14
Number of Residues	114
Details	TRANSMEM: Helical; Name=12

Chain	Residue	Details
A	ALA999-ALA1018
B	ALA999-ALA1018
C	ALA999-ALA1018
D	ALA999-ALA1018
F	ALA999-ALA1018
E	ALA999-ALA1018

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)