7O2I
METTL3-METTL14 heterodimer bound to the SAM competitive small molecule inhibitor STM2457
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 64.094, 64.094, 225.302 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.795 - 3.000 |
Rwork | 0.186 |
R-free | 0.26260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5l6d |
RMSD bond length | 0.011 |
RMSD bond angle | 1.287 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.795 | 3.180 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 1.800 | |
Rmeas | 0.274 | |
Number of reflections | 11480 | 1804 |
<I/σ(I)> | 12.2 | 2.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 19.7 | 15 |
CC(1/2) | 0.996 | 0.697 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 150 mM magnesium acetate, 22% w/v PEG 3350 |