7NX3
Crystal structure of ALK in complex with Fab324
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-01 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.8731 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 109.700, 137.470, 144.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.040 - 2.810 |
| R-factor | 0.2291 |
| Rwork | 0.228 |
| R-free | 0.26640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nuz |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.867 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.040 | 2.960 |
| High resolution limit [Å] | 2.800 | 2.810 |
| Rmeas | 0.250 | 1.200 |
| Number of reflections | 55385 | 6835 |
| <I/σ(I)> | 7.7 | |
| Completeness [%] | 99.0 | |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 287 | 40mMPolyamines, 0.1M Gly-Gly/AMPD pH8.5 12.5% w/v PEG4000 20% w/v 1,2,6-Hexanetriol |
