7NX0

LTK:ALKAL1 complex stabilized by a Nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-01
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9800
Spacegroup name	P 61
Unit cell lengths	129.110, 129.110, 109.750
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.260 - 1.950
R-factor	0.1703
Rwork	0.169
R-free	0.19580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7nx1
RMSD bond length	0.005
RMSD bond angle	0.899
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.300	2.000
High resolution limit [Å]	1.950	1.950
Rmeas	0.076	1.470
Number of reflections	75541	5362
<I/σ(I)>	26.6
Completeness [%]	99.9
Redundancy	20.8
CC(1/2)	0.990	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		287	MOPSO/BIS-TRIS pH 6.5 20% PEG 8000 40% w/v 1,5-pentanediol 5mM Na2CrO4 . 4H2O 5mM Na2MoO4 . 4H2O 5mM Na2WO4 . 4H2O 5mM Na2VO4 . 4H2O