7NUD
Crystal structure of mouse PRMT6 in complex with inhibitor EML734
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-30 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.766, 118.147, 71.998 |
| Unit cell angles | 90.00, 104.32, 90.00 |
Refinement procedure
| Resolution | 39.500 - 1.650 |
| R-factor | 0.1977 |
| Rwork | 0.197 |
| R-free | 0.21390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c03 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.080 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.120 | 2.058 |
| Rmeas | 0.123 | 2.215 |
| Rpim | 0.028 | 0.802 |
| Number of reflections | 81095 | 4071 |
| <I/σ(I)> | 14.9 | 1.1 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 18.5 | 7.3 |
| CC(1/2) | 0.999 | 0.479 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | PEG 3,350 20%, NaNO3 200 mM, Tris-HCl pH 7.5 100 mM |
