7NPI

Crystal structure of Mindy2 (C266A) in complex with Lys48-linked penta-ubiquitin (K48-Ub5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-25
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.965459
Spacegroup name	C 1 2 1
Unit cell lengths	162.061, 203.071, 265.035
Unit cell angles	90.00, 107.14, 90.00

Refinement procedure

Resolution	126.630 - 2.810
R-factor	0.2314
Rwork	0.228
R-free	0.28770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6z7v
RMSD bond length	0.003
RMSD bond angle	0.567
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	BALBES
Refinement software	PHENIX (1.19rc7_4070)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	126.630	3.223
High resolution limit [Å]	2.805	2.805
Number of reflections	88232	4412
<I/σ(I)>	6.2
Completeness [%]	87.8
Redundancy	2.3
CC(1/2)	0.995	0.741

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	3% w/v Dextran sulphate sodium salt 0.1 M BICINE pH 8.5 15% w/v PEG 20,000