7NE6

Human TET2 in complex with unfavourable DNA substrate.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	C 2 2 21
Unit cell lengths	47.858, 87.943, 261.988
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.970 - 2.300
R-factor	0.1967
Rwork	0.195
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nm6
RMSD bond length	0.003
RMSD bond angle	0.571
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.970	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.117	0.846
Rmeas	0.126	0.940
Rpim	0.045	0.394
Number of reflections	24979	2337
<I/σ(I)>	13.1	1.6
Completeness [%]	99.2	93.3
Redundancy	7.6	5.2
CC(1/2)	0.997	0.606

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	278	0.1M MES pH=6.3, 23% PEG2000MME