7NE1

Structure of the complex between Netrin-1 and its receptor Neogenin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97630
Spacegroup name	C 1 2 1
Unit cell lengths	150.950, 49.600, 157.250
Unit cell angles	90.00, 99.38, 90.00

Refinement procedure

Resolution	74.470 - 3.150
R-factor	0.214
Rwork	0.212
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bq6 1x5i 4plm
RMSD bond length	0.007
RMSD bond angle	0.890
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.570	3.230
High resolution limit [Å]	3.150	3.150
Rmerge	0.119
Rpim	0.118
Number of reflections	19817	1376
<I/σ(I)>	6.6	1
Completeness [%]	97.5
Redundancy	3.3
CC(1/2)	0.993	0.336

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298.15	0.2 M ammonium nitrate, 20% w/v PEG 3350, 40 mM potassium/sodium tartrate